Anti-5-Hydroxymethylcytosine [AB3/63.3] Standard size

Description: Recombinant monoclonal antibody to 5-Hydroxymethylcytosine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AB3/63.3.
Isotype and Format: Rabbit IgG, kappa
Clone Number: AB3/63.3
Published Application(s): IF; Dot; IP
Published Species Reactivity: Species independent
Immunogen: 5-Hydroxymethylcytosine conjugated to BSA.
Specificity: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine.
Application Notes: This antibody can be used to detect 5hmC by immunofluorescence and dot blot. Zhang et al. (2014, PMID: 24427299) used the original rat IgG2a format (under the name MABE176) in an immunoprecipitation experiment.
Antibody First Published in:
Ficz et al. Nature. 2011 May 19;473(7347):398-402 PMID:21460836 Note on publication: Describes the generation and use in immunofluorescence of the original antibody.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00862-23.0

Rabbit IgG

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Standard size 200 µg
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