Anti-MHC I [R1-21.2] Standard size

Description: Recombinant monoclonal antibody to MHC I. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R1-21.2. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Isotype and Format: Rat IgG2b, Fc Silent™, kappa
Clone Number: R1-21.2
Published Application(s): IHC; IP; radioimmunoassay
Published Species Reactivity: Mouse
Immunogen: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
Specificity: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
Application Notes: This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).
Antibody First Published in:
Koch et al Transplantation, August 1983, Vol.36(2), pp.177-80 [Peer Reviewed Journal] PMID:6192569 Note on publication: Describes the original generation and characterisation of this antibody.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00880-8.4

Rat IgG2b

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Standard size 200 µg
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