United Kingdom (UK)
Recombinant monoclonal antibody to alpha-V integrin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma EM01309.
UniProt Accession Number of Target Protein: P06756
Alternative Name(s) of Target: CD51; Vitronectin receptor subunit alpha; av
Immunogen: This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas
Specificity: This pan-αv antibody is specific for the extracellular domain (amino acids 31 - 992) of the αv chain in all αv-containing heterodimeric complexes.
Application Notes: This pan-αv-specific antibody has been used to detect αv integrin chains using ELISA and on Western blots (Goodman et al, 2012), and to specifically stain αv integrins in FFPE/TMA material (Goodman et al, 2012; Silginer et al, 2016; Roth et al, 2013). Murine antibodies are typically unable to detect FFPE-embedded integrins.
Antibody first published in:
Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423
Note on publication:
Describes the generation and characterisation of this antibody.
Immunohistochemical staining of various human tumour cell lines using anti-alpha-V integrin antibody (Ab00887) E01309 Anti-alpha-V integrin staining of formaldehyde fixed paraffin embedded Panc-1, PC-9 and Jurkat cell line samples exhibiting varying degrees of target expression (level in parentheses). The rabbit-chimeric version of E01309 (Ab00887-23.0) was used to stain samples at a concentration of 0.3 µg/ml.