United Kingdom (UK)
Recombinant monoclonal antibody to DARC. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2C3.
UniProt Accession Number of Target Protein: Q4VBN9
Alternative Name(s) of Target: Fy6; Duffy Antigen Receptor for Chemokines; NaM185-2C3
Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.
Specificity: The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.
Application Notes: The antibody binds to DARC, a glycoprotein chemokine receptor and the only known receptor for Plasmodium vivax.
Antibody first published in:
Wasniowska et al. Structural characterization of the epitope recognized by the new anti-Fy6 monoclonal antibody NaM 185-2C3. Transfusion Medicine 2002; 12(3):204-211 PMID:12164140
Note on publication:
Describes the characterization of of the 2C3-epitope and how the antibody inhibits interleukin-8 binding to the Duffy receptor.
Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (Ab00893-23.0) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. Ab00893-23.0 successfully detected DARC in human liver lysate.
Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3 (Ab00893) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (Ab00893-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00893-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.