Anti-CTLA-4 [9H10]

Description:

Recombinant monoclonal antibody to CTLA-4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9H10.

Clone Name:

9H10

Published Application(s):

Neutralisation, proliferation assays, WB, ELISA, FC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab00894-2.0 Anti-CTLA-4 [9H10]
Mouse
Mouse IgG2a
Purified
Ships in 4-5 weeks

Ab00894-2.3 Anti-CTLA-4 [9H10]
Mouse
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab00894-23.0 Anti-CTLA-4 [9H10]
Mouse
Rabbit IgG
Purified
In Stock

Ab00894-1.32 Anti-CTLA-4 [9H10]
Mouse
Mouse IgG1-D265A
Fc Silenced
Purified
Ships in 4-5 weeks

Ab00894-22.0 Anti-CTLA-4 [9H10]
Mouse
Hamster (Armenian) IgG
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P09793

Alternative Name(s) of Target: CD152; CTLA4; cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte protein 4;
Immunogen: This antibody was raised by immunising Syrian hamsters with Staphylococcus A bacteria coated in CTLA-4, isolating B cells from the immunised hamsters and fusing these with the P3X3.Ag8.653 myeloma line to produce stable hybridomas.

Specificity: This antibody is specific for murine CTLA-4, an inhibitory receptor that acts as the primary negative regulator of T-cell responses. CTLA-4 is expressed predominantly by activated T cells, with significantly higher levels of expression on CD8+ T cells than CD4+ T cells.

Application Notes: CTLA-4 is upregulated on T cells following their activation, and acts as a negative regulator of T cell responses; CTLA-4 binds to the B7 molecules CD80 and 86, resulting in the delivery of an inhibitory signal, and consequent downregulation of T cell-mediated immunity. Administration of 9H10 blocks the interaction between CTLA-4 on the T cell surface and CD80 and CD86. This promotes the activation of effector T cells and stimulates the immune response raised against weak antigens, including tumour antigens. While this antibody alone does not enhance T cell proliferation, it does significantly increase T cell proliferation when administered together with anti-CD28 (clone 37.51) (Krummel & Allison, 1995), anti-OX40 and anti-GITR (Houot & Levy, 2009). Blocking CTLA-4 induces T cell anti-tumour immunity in animal models, both by suppressing regulatory T cell activity and directly promoting CD8+ T cell effector function (Peggs et al, 2009). In transgenic murine models of prostate cancer, the use of a CTLA-4 blockade in conjunction with an irradiated tumour cell vaccine stimulates an immune response against primary tumours, and results in a significant reduction in tumour incidence (Hurwitz et al, 2000). Similarly, in murine melanoma models, CTLA-4 blockage, in combination with CD40 stimulation and adenoviral vaccination, can elicit complete regression (Sorensen et al, 2010). In murine models of pancreatic ductal adenocarcinoma, 9H10 has also been shown to induce T cell-dependent tumour regressions (Vonderheide et al, 2015). Priming the T cell response with CD40 mAbs or chemotherapy reversed the resistance to 9H10 and RMP1-14 observed in well-established tumours. Additionally, this antibody has been used to detect CTLA-4 using ELISA (Krummel & Allison, 1995) and to stain CTLA-4-expressing cells (Deeths et al, 1999).

Antibody first published in: Krummel & Allison CD28 and CTLA-4 Have Opposing Effects on the Response of T cells to Stimulation J Exp Med. 1995 Aug 1; 182(2): 459–465. PMID:7543139
Note on publication: Describes the original generation and characterisation of this antibody.

ELISA using Ab00894-23.0. Click on image for more details.

ELISA of anti-CTLA-4 antibody on mouse CTLA-4-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (Ab00894-23.0; red line) and isotype control (anti-Fluorescein antibody Ab00102-23.0 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein (Pr00155-1.9) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using Ab00894-23.0. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.

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