United Kingdom (UK)
Recombinant monoclonal antibody to TCR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Desire-1.
UniProt Accession Number of Target Protein: P01852; P01849
Alternative Name(s) of Target: T cell receptor; T-cell receptor; Ti
Immunogen: mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
Specificity: mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
Application Notes: Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
Antibody first published in:
Hua et al. Lysis of hybridoma cells bearing anti-clonotypic surface immunoglobulin by clonotype-expressing alloreactive cytotoxic T cells Eur. J. Immunol. 1985. 15: 1029-1032 PMID:3902483
Note on publication:
Describes the ability desire-1 to modulate the cytolytic activity of cytotoxic T lymphocyte clone KB5-C20.
Immunofluorescence staining of mouse splenocytes using anti-TCR antibody (Ab00904) Desire-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of Desire-1 (Ab00904-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00904-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.