Anti-IL-2 [Jes6-1] Standard size

Description: Recombinant monoclonal antibody to IL-2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Jes6-1. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Isotype and Format: Mouse IgG2a, Fc Silent™, kappa
Clone Number: Jes6-1
Published Application(s): ELISA; IP; NTRL
Published Species Reactivity: Mouse
Immunogen: Rat antibodies were raised against murine IL-2.
Specificity: This antibody binds murine IL-2.
Application Notes: JES6-1 is a scFv reformat of the variable domain sequences of the JES6-1 monoclonal antibody (Spangler et al, 2015). It was shown in this study that JES6-1 scFv binding to mouse IL-2 would sterically hinder interaction with the IL-2 receptor, thereby blocking downstream signalling functions. This antibody is well known as a neutralising antibody against mouse IL-2, and is useful for cytokine assays such as ELISA (Abrams et al, 1992).
Antibody First Published in:
Abrams et al. Immunological Reviews 1992, No. 127 PMID:1387110 Note on publication: Used the anti-mouse IL-2 clone JES6-1A12 to assay the presence of mouse IL-2 in enzyme-linked assays.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00913-2.3

Mouse IgG2a

Ships in 2-3 weeks
Standard size 200 µg
Custom conjugation:
Clear selection
Ships in 2-3 weeks
Bulk size
1 mg