United Kingdom (UK)
Recombinant monoclonal antibody to CD10. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NL-1.
UniProt Accession Number of Target Protein: P08473
Alternative Name(s) of Target: Neprilysin; Cluster of Differentiation 10; Membrane metallo-endopeptidase; MME; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP; Common acute lymphocytic leukaemia antigen; CALLA; Skin fibroblast elastase; SFE; CMT2T; SCA43; Atriopeptidase; Enkephalinase
Immunogen: BALB/c mice were immunised with acute lymphocytic leukaemia cells from a human leukaemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma.
Specificity: This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia.
Application Notes: NL-1 was shown to react with several leukaemic cell lines using immune adherence assays (Ueda et al, 1982). These authors also confirmed that NL-1 is specific for CD10 using IP.
Antibody first published in:
Ueda et al. Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4386-90. PMID:6956869
Note on publication:
Describes the generation and characterisation of a panel of mAbs against various leukaemic cell lines with the goal of using this panel of mAbs to define blood antigen systems.
Immunofluorescence staining of fixed Jurkat cells with anti-CD105 antibody NL-1 (Ab00950) Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of NL-1 (Ab00950-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing punctate cytoplasmic staining, likely from protein trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00950-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.