United Kingdom (UK)
Recombinant monoclonal antibody to Eimeria acervulina. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5D11.
Alternative Name(s) of Target: 5D-11
Immunogen: This antibody was raised by immunising chickens with CD8+ T cells pre-adsorbed with E. acervulina soluble sporozoite antigen.
Specificity: This antibody recognises an antigen on the apical tip of E. acervulina and E. maxima sporozoites.
Application Notes: This antibody has been used in confocal laser immunofluorescence analysis to stain Eimeria acervulina and E. maxima sporozoites, but will not stain merezoites (Constantinoiu et al, 2003). An scFv derived from the variable regions of this antibody has been used in ELISA, IFA and immunoblot assays (Kim et al, 2001)
Antibody first published in:
Kim et al. Generation and Characterization of Recombinant ScFv Antibodies Detecting Eimeria acervulina Surface Antigens Hybridoma. 2001 Jun;20(3):175-81. PMID:11461666
Note on publication:
Describes the derivation of an scFv from this monoclonal antibody.