United Kingdom (UK)
Recombinant monoclonal antibody to Eimeria acervulina. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8C3.
UniProt Accession Number of Target Protein: Q24769
Alternative Name(s) of Target: 8C3HLEK; Eimeria acervulina sporozoite
Immunogen: The original antibody was generated by immunizing chickens with coccidial antigens prepared from Eimeria acervulina sporozoites.
Specificity: This antibody is specific for an unidentified surface antigen localized in the apical complex of Eimeria acervulina, a protozoan causing coccidiosis in poultry.
Application Notes: The original version of this antibody (chicken IgG, λ) antibody was generated and screened against surface antigens of Eimeria acervulina using ELISA (Song et al., 2001) (Kim et al., 2001; US6790937B2). A recombinant scFv version of this antibody, expressable in E. coli, was generated, screened via ELISA and characterized (Park et al., 2005; PMID: 15834788).
Antibody first published in:
Song et al. Molecular Cloning and Characterization of CDNA Encoding Immunoglobulin Heavy and Light chain Variable Regions from Four Chicken Monoclonal Antibodies Specific to Surface Antigens of Intestinal Parasite, Eimeria acervulina J. Microbiol. 2001 Mar 1. 39(1):49-55 PMID:15834788
Note on publication:
The original publication describes the molecular cloning and characterization of cDNA encoding immunoglobulin heavy and light chain variable regions from four chicken monoclonal antibodies developed to combat avian coccidiosis, caused by the intestinal parasite Eimeria acervulina, employing antibody engineering technology to overcome limitations associated with the hybridoma method.