United Kingdom (UK)
Recombinant monoclonal antibody to CpaF. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display 556.
UniProt Accession Number of Target Protein: C6XFP4
Alternative Name(s) of Target: Component of type IV pilus
Immunogen: BALB/c mice were immunised by injection of a high titre of Liberibacter asiaticus obtained from infected Diaphorina citri psyllids. The spleens of immunised mice were removed and homogenised for RNA extraction and cDNA synthesis.
Specificity: Clone 556 was shown to bind purified CpaF by ELISA (Yuan et al, 2015).
Application Notes: Clone 556 was tested for Liberibacter asiaticus recognition as a phage-expressing scFv using blotting (Yuan et al, 2015). This format was also tested against specific recognition of the antigen CpaF using ELISA.
Antibody first published in:
Yuan et al. Development of single chain variable fragment (scFv) antibodies against surface proteins of 'Ca. Liberibacter asiaticus'. J Microbiol Methods. 2016 Mar;122:1-7. PMID:26744234
Note on publication:
Describes the generation and brief characterisation of anti-Liberibacter asiaticus scFv-formatted antibody fragments specific for different antigens.