Anti-PD-1H [MH5A] Standard size

Description: Recombinant monoclonal antibody to PD-1H. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MH5A.
Isotype and Format: Mouse IgG1, lambda
Clone Number: MH5A
Published Application(s): ELISA; IHC; block; therapeutic
Published Species Reactivity: Mouse
Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
Specificity: This antibody specifically binds PD-1H.
Application Notes: The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-μg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).
Antibody First Published in:
Flies D et al. J Immunol August 15, 2011, 187 (4) 1537-1541 PMID:21768399 Note on publication: Describes the generation of the antibody and characterisation of its theraputic function in models of graft-versus-host disease.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab01016-1.1

Mouse IgG1

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Standard size 200 µg
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