CD45 (leukocyte common antigen, LCA) is a surface antigen notable for its presence on all differentiated hematopoietic cells, with the exception of erythrocytes. Anti-CD45 antibodies are a common tool in flow cytometry to aid in the identification of cells, and are also used to distinguish lymphomas and myelomas from carcinomas in the clinic.
An exciting prospect is the possibility of using antibodies targeting CD45 to deplete cells of the immune system. For patients in need of a bone marrow transplant, this would be preferable to the harmful radiation and chemical immunosuppressants currently used for conditioning.
| Antigen | Antibody Clone | Product Code |
|---|---|---|
| human CD45 | YTH24.5 | Ab00671 |
| human CD45 | YTH54.12 | Ab00672 |
| human CD45 | YTH 80.103 | Ab00196 |
| human CD45RO | UCH-L1 | Ab00604 |
| canine CD45R | YKIX 753.22.2 | Ab00598 |
| murine CD45 | YW 62.3 | Ab00204 |
Our collection of anti-CD45 antibodies includes popular clones YTH54.12 and YTH24.5 which bind non-overlapping epitopes. Used in combination in their original rat IgG2b format, they were shown to successfully deplete cells of the hematopoietic lineage in humans (PMID 241487).
Because of their recombinant manufacture, all our products benefit from minimal batch-to-batch variation, as well as options for customisation. All antibodies are available in a range of species and formats, meaning our customers can ’build’ the reagent that works for them.
For example, YTH54.12 and YTH24.5 are available in human IgG1 and rabbit IgG formats as well as the original rat IgG2b. Our chimeric rabbit antibodies are especially popular due to their low background staining and compatibility with a different set of secondary reagents. The below data show that engineered rabbit versions of YTH24.5 and YTH54.12 clones maintain strong activity.
Flow-cytometry data for clones YTH24.5 and YTH54.12. Human leukocytes were stained with an antibody isotype control (panel A) and the rabbit-chimeric versions of YTH24.5 (panel B) and YTH54.12 (panel C). Bound antibody was detected using the same AF488-conjugated donkey anti-rabbit secondary reagent.
Can’t see what you’re looking for? Or perhaps you can suggest an antibody to add to our collection? Get in touch – we are always happy to hear from you.
