Our FleXpress™ high-throughput recombinant antibody production service offers clients the flexibility to rapidly express a large number of IgG antibodies at 80 ml scale using Absolute Antibody’s proven recombinant antibody technology. The service utilizes our HEXpress™ antibody expression platform, a serum-free, mammalian transient expression system, to manufacture high-quality recombinant antibodies with high purity and low endotoxin levels.
The FleXpress™ production line can recombinantly manufacture hundreds of different antibodies per week at a fixed volume of 80 ml, allowing customers to quickly and cost-effectively test recombinant versions of many different antibodies. Purification is by a fully automated, custom-built system capable of purifying up to 24 antibodies at a scale of 80 ml in approximately 12 hours. This service is particularly useful for the following applications:
- Multi-hybridoma conversion: reagent companies with a monoclonal antibody portfolio can test moving their collection from hybridomas to recombinant production, to take advantage of the many benefits of recombinant expression, including superior batch-to-batch reproducibility, antibody engineering options, and guaranteed long-term supply. Learn more about our multi-hybridoma conversion service here.
- Early-stage biopharmaceutical R&D: therapeutic antibody developers can quickly screen or conduct pilot studies on a group of therapeutic antibody candidates by taking advantage of the FleXpress™ platform’s affordable high-throughput protein production capabilities.
All antibody production occurs in our ISO-certified antibody manufacturing facility, which also houses two other production lines enabling scale-up to multi-gram quantities in a matter of weeks, for clients in need of larger-scale manufacturing. In 2020, we used our production platform to produce thousands of different recombinant antibodies, making 180 grams of protein in total.
In addition, we’ve used our production platform to build a unique recombinant reagents catalog consisting of more than 8,500 antibodies, all benefitting from antibody engineering technology. For example, check out one catalog clone (anti-EGFR, or Cetuximab) available in more than 30 different off-the-shelf formats. The FleXpress™ production service enables our customers to utilize this same technology for their own recombinant antibody projects.
To learn more, contact us or fill out the form below to start a discussion about your project.