Hybridoma Sequencing

Absolute Antibody offers a quick and professional hybridoma sequencing service, also known as antibody sequencing. Hybridoma sequencing is the sequencing of the cDNA encoding the variable heavy (VH) and variable light (VL) domains of your hybridoma cell line.

Sequencing the antibody genes of your hybridoma offers many advantages:

  • Security – your antibody can always be produced recombinantly even if your hybridoma line is lost
  • Humanization – once you know the VH and VL sequences you can proceed to humanization if part of a therapeutic antibody project
  • Flexibility – convert your antibody to any species, isotype or format
  • Intellectual property – patent the sequences of your unique variable domains

Contact us to receive a quote or to discuss your requirements in more detail.

Guaranteed Antibody Sequencing Service

5,000+ hybridomas sequenced.

Our method – The mRNA transcriptome is obtained from the hybridoma and random hexamers are used to generate a cDNA library. This is fragmented, and undergoes paired-end sequencing. The sequencing reads are assembled into contigs, from which antibody transcripts are identified based on homology.

At Absolute Antibody we perform high throughput (NGS) hybridoma sequencing. In 4-6 weeks, we deliver you all VH and VL sequences as well as a full report characterizing your hybridoma’s sequences and any heterogenicity or other antibody isotypes that might be present.

  • Works on any species or isotype
  • Use of random primers means that no mutations are introduced
  • Identifies all HC and LC sequences and quantifies their relative abundance
  • No sequence, no fee for any viable hybridoma
  • Your sequence is your property – all our services are royalty-free
  • High-throughput method can sequence up to 50 hybridomas concurrently – bulk hybridoma sequencing packages available
  • Sequence delivered in 4-6 weeks

Even if a hybridoma is contaminated or unproductive, we could be able to save your antibody for recombinant expression. Review our collection of hybridoma sequencing case studies to learn how our unique NGS method can help overcome such obstacles to antibody production. Contact us about your project to see if we can help.

If you want to sequence a large hybridoma collection or your entire antibody catalog, contact us to discuss our bulk hybridoma sequencing options.

If you want to sequence antibody directly from purified protein, read about our protein sequencing service.

We also provide follow on services – read our antibody engineering and antibody expression pages for more information.

We provide instructions for shipping hybridoma cell lines in your order quote. You may request a free SeqPack™ Hybridoma Preservation Kit if you do not wish to ship using dry ice.

Please contact us to learn more about our hybridoma sequencing and request an order quote. Bulk discounts may apply.

Interested in more information about hybridoma sequencing? Check out our blog post: Why Your Hybridoma Should be Sequenced with NGS Instead of PCR.