Recombinant Antibodies derived from Human Patients

Human antibodies from patients are being sequenced at an increasing rate – see Quake et al. for an up to date review of high throughput sequencing of antibodies. Amongst others, we are excited to offer you a range of human-derived antibodies specific to viral pathogens or self-antigen (many in collaboration with Humabs BioMed SA, Bellinzona, Switzerland). Our proprietary cloning platform allows us to produce high quality recombinant versions of these antibodies – we think some will be valuable reagents for research and diagnostics. We offer the original human IgG version as well as a reformatted mouse and rabbit versions to suit your experimental requirements- contact us for bulk custom orders. For help on their applications see our application codes page.

Adjacent figure shows: Indirect immunofluorescence (IF) staining of live keratinocyte monolayers with pemphigus vulgaris (PV) auto-antibodies
(A) Cryosections of human skin stained with biotinylated DSG3-specific monoclonal antibodies (Ab00153, Ab00155, Ab00158 and Ab00156).
(B) Staining of human epidermis with biotinylated PVB28 (Ab00158) in the presence (with) or absence (w/o) of Ca2+.
(C) Binding of a panel of pemphigus auto-antibodies to DSG3-coated ELISA plates in the presence or absence of EDTA.Antibodies described in (external links): Corti D et al. 2010 as well as Di Zenzo G et al. 2012

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Recombinant Antibodies derived from Human Patients

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