Anti-A14 [BB5.2]

Description:

Recombinant monoclonal antibody to A14. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BB5.2.

Clone Name:

BB5.2

Published Application(s):

IP, WB, IF

Published Species Reactivity:

Vaccinia virus

Products based on this clone:

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Ab00733-1.1 Anti-A14 [BB5.2]
Vaccinia virus
Mouse IgG1
Purified
Ships in 4-5 weeks

Ab00733-23.0 Anti-A14 [BB5.2]
Vaccinia virus
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: N/A

Alternative Name(s) of Target: Vaccinia A14 phoshoprotein; Vaccinia virus A14 phosphoprotein; virion membrane protein A14
Immunogen: This antibody was produced by infecting a BALB/c mouse intranasally with WR.K1L–C7L–, a highly attentuated vaccinia virus. The mouse was subsequently challenged with intranasal inoculation of of wild type VACV, followed by intravenous injection with UV-inactivated VACV.

Specificity: This antibody is specific for the vaccinia virus A14 phosphoprotein, which is expressed on the membranes of intracellular mature virions.

Application Notes: A14 was among one of a group of VAVC proteins that elicited strong polyclonal antibody responses, resulting in multiple clones of hybridomas being generated against the same protein, all of which were analysed with IF, IP and WB. These clones have not, as of yet, been individually described in published literature.

Antibody first published in: Meng et al. Generation and characterization of a large panel of murine monoclonal antibodies against vaccinia virus Virology, volume 409, Issue 2, 20 January 2011, Pages 271–279 PMID:21056889
Note on publication: Describes the original generation of a panel of antibodies capable of recognizing VACV antigens, including A14.

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