Anti-BSA [D1]

Description:

Recombinant monoclonal antibody to BSA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody D1.

Clone Name:

Published Application(s):

Published Species Reactivity:

Bovine

Products based on this clone:

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Ab00748-1.1 Anti-BSA [D1]
Bovine
Mouse IgG1
Purified
In Stock

Ab00748-23.0 Anti-BSA [D1]
Bovine
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: P02769

Alternative Name(s) of Target: Bovine serum albumin; serum albumin; albumin
Immunogen: D1 was prepared by isolating anti-p24 scFvs from a phage display library made from a mouse immunized with lysate from HIV-1 infected H9 cells.

Specificity: D1 has been shown to bind to BSA in panning experiments from phage display. Albumin is a secreted globular protein found in blood plasma and BSA has multiple biochemical applications including ELISAs and immunohistochemistry and is able to increase the stability of proteins and be used as a standard in Bradford assays.

Application Notes: D1 was selected for by panning where it reacted postiively on BSA. D1 can also be used as a control in IP and when working with native proteins when conducting studies within a BSA medium.

Antibody first published in: de Haard et al. Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics. Clin Diagn Lab Immunol. 1998 Sep;5(5):636-44. PMID:9729530
Note on publication: Describes the production of the anti-BSA antibody D1 in addition to antibodies specific for the viral protein p24.

ELISA using Ab00748-23.0. Click on image for more details.

ELISA of anti-BSA antibody on BSA. Binding curves of the rabbit IgG chimeric version of the anti-BSA antibody D1 (Ab00748-23.0; blue line) and isotype control (Ab00102-23.0, anti-fluorescein; orange line) to an ELISA plate coated with BSA at a concentration of 5 µg/ml. A 3-fold serial dilution from 90 µg/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an HRP-conjugated anti-rabbit IgG antibody was used.

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