Anti-CD155 [3F1]

Description:

Recombinant monoclonal antibody to CD155. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F1.

Clone Name:

3F1

Published Application(s):

ELISA, FC

Published Species Reactivity:

Mouse

Products based on this clone:

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Ab01298-7.1 Anti-CD155 [3F1]
Mouse
Rat IgG2a
Purified
In Stock

Ab01298-7.4 Anti-CD155 [3F1]
Mouse
Rat IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01298-23.0 Anti-CD155 [3F1]
Mouse
Rabbit IgG
Purified
In Stock

Ab01298-2.0 Anti-CD155 [3F1]
Mouse
Mouse IgG2a
Purified
In Stock

Ab01298-2.3 Anti-CD155 [3F1]
Mouse
Mouse IgG2a
Fc Silent™
Purified, VivopureX
In Stock

Ab01298-1.1 Anti-CD155 [3F1]
Mouse
Mouse IgG1
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: Q8K094

Alternative Name(s) of Target: TAGE 3F1; Poliovirus receptor; cellular receptor for poliovirus; Pvr; Tumor-associated antigen 1; Taa1; Tage4; HVED
Immunogen: This antibody was raised by immunising rats with recombinant murine CD155, consisting of the entire ectodomain expressed as a HIS-tagged protein.

Specificity: This antibody is specific for murine CD155.

Application Notes: The binding specificity of this antibody has been confirmed both in ELISA analysis using recombinant murine CD155 (mCD155), and in flow cytometric analysis using HEK293 cells transiently expressing mCD155 (Maier et al, 2007). This antibody has been used in flow cytometry to assess mCD155 expression on lymphocytes and dendritic cells (Maier et al, 2007; Seth et al, 2011). This antibody blocks the interaction of CD155 with its ligands CD96 and CD226 (Qiu et al, 2010). When administered to WT mice, antibody-induced disruption of the CD155-CD226 interaction has been shown to cause CD226 upregulation on CD4+ T cells and CD8+ T cells (Seth et al, 2011). Administration of this antibody has also been shown to stimulate the loss of mature, memory-like CD8+ T cells from the thymus (Seth et al, 2011; Georgiev et al, 2016).

Antibody first published in: Maier et al. The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens Eur J Immunol. 2007 Aug;37(8):2214-25. PMID:17621371
Note on publication: Describes the original generation of this antibody, as part of a panel of anti-CD155 antibodies.

WB on mouse thymus lysate using Ab01298-23.0. Click on image for more details.

Western Blot using anti-CD155 antibody 3F1 (Ab01298). Mouse thymus lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 3F1 (Ab01298-23.0) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF analysis of RAW 264.7 cells using Ab01298-23.0. Click on image for more details.

Immunofluorescence staining of RAW 264.7 cells using anti-CD155 antibody (Ab01298) 3F1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3F1 (Ab01298-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01298-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.

ELISA using Ab01298-23.0. Click on image for more details.

Binding curve of anti-CD155 antibody 3F1 (Ab01298-23.0) to recombinant mouse CD155 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse CD155 Fc-Fusion Protein (Pr00231-1.9; Absolute Antibody) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using Ab01298-23.0. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.

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