United Kingdom (UK)
Recombinant monoclonal antibody to CD3 epsilon. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 500A2.
UniProt Accession Number of Target Protein: P22646
Alternative Name(s) of Target: CD3e; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
Immunogen: T cell receptor complexes derived from mouse C6VL-BS thymoma cells (C57BL/Ka) .
Specificity: The 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains.
Application Notes: This antibody binds to murine CD3 epsilon. It partially cross-blocks binding of clone 145-2C11 (Ab00112) indicating that overlapping epitopes are detected by these antibodies.
Antibody first published in:
Allison, J. P. The T Cell Receptor UCLA Symposia on Molecular and Cellular Biology, New Series (eds Kappler, J. & Davies, M.) (Lissabon, New York, 1987) PMID:n/a
Note on publication:
Describes the making of the hamster monoclonal antibodies directed against the epsilon chain of murine CD3.
Flow-cytometry using anti-CD3epsilon antibody 500A2 (Ab00121) Mouse lymphocytes were stained with an anti-CD44 control (Ab00628-23.0, panel A) or the rabbit-chimeric version of 500A2 (Ab00121-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.