United Kingdom (UK)
Recombinant monoclonal antibody to CD43. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma W3/13.
UniProt Accession Number of Target Protein: P13838
Alternative Name(s) of Target: Leukosialin; sialophorin; Leukocyte sialoglycoprotein; Ly-48; Ly48
Immunogen: Rat thymocyte membrane glycoproteins injected into BALB/c mice.
Specificity: This antibody binds to CD43, which is expressed on lymphocytes.
Application Notes: This antibody detects CD43 on lymphoid cells, and can be used to label thymocytes, lymphocytes from the bone marrow and thoracic duct lymphocytes (Williams et al, 1977). Similarly, W3/13 can be used in immunohistochemical staining, enabling the elucidation of the cellular organisation of lymph nodes (Barclay, 1981).
Antibody first published in:
Williams et al. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: Differentiation antigens of rat lymphocytes Cell, 1977, Vol.12(3), pp.663-673 PMID:303545
Note on publication:
Describes the original use of this antibody to detect CD43 in lymphoid cell labelling studies
Flow-cytometry using anti-CD43 (Leukosialin) antibody W3/13 (Ab00540) Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or the rabbit-chimeric version of W3/13 (B, Ab00540-23.0) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.
Western Blot using anti-CD43 (Leukosialin) antibody W3/13 (Ab00540) Rat spleen (A), thymus (B) and lymph node (C) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of W3/13 (Ab00540-23.0) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD43 is 100-120kDa. Ab00-23.0 successfully detected CD43 in rat spleen, thymus and lymph node samples.