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- Ab00868-23.0 Anti-CD49b [16B4]
- Human
- Rabbit IgG
- Purified
- In Stock
- Ab00868-13.12 Anti-CD49b [16B4]
- Human
- Human IgG4-S228P
- Purified
- Ships in 4-5 weeks
- Ab00868-1.1 Anti-CD49b [16B4]
- Human
- Mouse IgG1
- Purified
- In Stock
Recombinant monoclonal antibody to CD49b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 16B4.
UniProt Accession Number of Target Protein: P17301
Alternative Name(s) of Target: Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; alpha 2 subunit of VLA-2 receptor; BR; CD49B; human platelet alloantigen system 5; ITGA2; platelet antigen Br; platelet glycoprotein GPIa; very late activation protein 2 receptor, alpha-2 subunit
Specificity: This antibody is specific for the α2 integrin subunit, and recognises an epitope outside the von Willebrand factor A-type, or collagen-binding domain. Integrins are heterodimeric integral membrane glycoproteins, which are expressed by a range of cells, including T cells, NK cells, fibroblasts and platelets, where they mediate cell adhesion.
Application Notes: This antibody has been used in flow cytometric analysis to investigate the surface expression of α2 integrin by cultured human endothelial cells (Sincock et al, 1999), and in immunoprecipitation analysis to assess the interaction between PETA-3 and integrins on the surface of endothelial cells (Sincock et al, 1999). Alexa Fluor 647-conjugated 16B4 has been used to evaluate the expression of integrin α2β1 on the surface of platelets by confocal imaging (Poulter et al, 2017). This antibody has been employed to detect integrin α2β1 expression through in-cell Western blotting analysis in primary fibroblasts (Suarez et al, 2014). This antibody has also been used to coat polystyrene beads as part of a microsphere-based cellular adhesion assay; human osteosarcoma cells transfected with the integrin a2 subunit displayed high levels of interaction with beads coated in this anti-α2 integrin antibody (Connors & Heino, 2004).
Antibody first published in:
Sincock et al. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function Journal of Cell Science 112, 833-844 (1999) PMID:10036233
Note on publication:
Describes the original use of this antibody in flow cytometric and immunoprecipitation analysis.
Immunofluorescence staining of A431 cells using anti-CD49b antibody (Ab00868) 16B4. Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of 16B4 (Ab00868-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00868-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-CD49b antibody 16B4 (Ab00868). A431 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 16B4 (Ab00868-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.