Anti-CD52 [HI186 ]

Description:

Recombinant monoclonal antibody to CD52. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HI186 .

Clone Name:

HI186

Published Application(s):

IHC-P, FC

Published Species Reactivity:

Human

Products based on this clone:

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Ab00706-10.0 Anti-CD52 [HI186 ]
Human
Human IgG1
Purified
In Stock

Ab00706-23.0 Anti-CD52 [HI186 ]
Human
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: P31358

Alternative Name(s) of Target: CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5
Immunogen: Human tonsil cells.

Specificity: This antibody binds to human CD52.

Application Notes: This antibody binds to human CD52.

Antibody first published in: Rodig et al. Heterogeneous CD52 Expression among Hematologic Neoplasms: Implications for the Use of Alemtuzumab (CAMPATH-1H). Clin Cancer Res. 2006 Dec 1;12(23):7174-9. PMID:17145843
Note on publication: Describes the use of this antibody in IHC.

IF staining of Daudi cells using Ab00706-23.0. Click on image for more details.

Immunofluorescence staining of Daudi cells with anti-CD52 (Ab00706) HI186 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HI186 (Ab00706-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00706-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot on human spleen tissue lysate using Ab00706-23.0. Click on image for more details.

Western Blot using Anti-CD52 antibody HI186 (Ab00706). Human spleen tissue lysate (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HI186 (Ab00706-23.0) 1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry analysis of Daudi cells using Ab00706-23.0. Click on image for more details.

Flow cytometry using the Anti-CD52 antibody HI186 (Ab00706). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of HI186 (Ab00706-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of Daudi cells using Ab00706-23.0. Click on image for more details.

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