United Kingdom (UK)
Recombinant monoclonal antibody to CD59. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YTH 53.1.
UniProt Accession Number of Target Protein: P13987
Alternative Name(s) of Target: MAC-IP; CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; HRF-20; MAC-inhibitory protein; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; YTH53; YTH-53
Immunogen: T lymphocytes in human PBMC.
Specificity: Recognises CD59 18-20kD cell surface GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. This protein is regulator of complement-mediated cell lysis by inhibiting the complement membrane attack complex formation of C8 and/or C9.
Antibody first published in:
Davies A, Simmons DL, Hale G, Harrison RA, Tighe H, Lachmann PJ, Waldmann H. CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J Exp Med. 1989 Sep 1;170(3):637-54. PMID:2475570
Note on publication:
Describes the preparation of antibody and characterisation of the specificities by immunofluorescence and flow cytometry and complement-mediated cell lysis assays.
Flow-cytometry using anti-CD59 antibody YTH 53.1 (Ab00197) Human leukocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YTH 53.1 (Ab00197-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.