United Kingdom (UK)
Recombinant monoclonal antibody to CD70. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BU69.
UniProt Accession Number of Target Protein: P32970
Alternative Name(s) of Target: TNFRS7; Tumor Necrosis Factor Ligand Superfamily Member 7; CD27 Ligand; CD27LG; TNFSF7; CD27L; Tumor Necrosis Factor Ligand 8A; Surface Antigen CD70; Ki-24 Antigen; CD70 Antigen; TNLG8A; TAN1-7; TAN17
Immunogen: Murine antibodies were produced by immunising BALB/c mice using the Waldenstrom's macroglobulinaemia cell line WM-1. Hybridomas were generated by fusion of immunised cells with the myeloma cell line P3/NS1/1-Ag4.1 via established methods.
Specificity: This antibody binds human CD70, which is the CD27 ligand. This protein is present only on activated dendritic cells and T lymphocytes, and represents a therapeutic target of relevance for autoimmune diseases.
Application Notes: BU69 has been used for FACS and immunocytochemistry analysis of the cell-surface expression levels of CD70 in renal cell carcinomas (Adam et al, 2006). It was shown in this study that cell-surface binding of BU69 to CD70 caused rapid internalisation of the cell-surface glycoprotein.
Antibody first published in:
Edited by Schlossman et al. Leukocyte typing V: White cell differentiation antigens Oxford PMID:
Note on publication:
Describes the repertoire of leukocyte differentiation antigens (i.e. Clusters of Differentiation, and other such cell-surface molecules), specifically using a panel of mAbs to then facilitate characterisation of distinct cell types based on their antibody reactivity patterns.
Western Blot using anti-CD70 antibody BU69 (Ab00818) Human spleen lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and the blot probed with the chimeric rabbit IgG version of BU69 (Ab00818-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD70 is 21.1kDa, but this protein is glycosylated at several positions and may form homotrimers.