United Kingdom (UK)
Recombinant monoclonal antibody to GITR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma T10P2H9*B9.
UniProt Accession Number of Target Protein: Q9Y5U5
Alternative Name(s) of Target: CD357; TNFRSF18; Tumor Necrosis Factor Receptor Superfamily, Member 18; TNFRSF18-soluble form; GITR; Glucocorticoid-induced TNFR-related protein
Specificity: This antibody is specific for the soluble isoform of TNFRSF18.
Antibody first published in: PMID:
Immunofluorescence staining of human peripheral blood monocytes using anti-GITR antibody (Ab00727) T10P2H9*B9. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of T10P2H9*B9 (Ab00727-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00727-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-GITR antibody T10P2H9*B9 (Ab00727). Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of T10P2H9*B9 (Ab00727-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.