United Kingdom (UK)
Recombinant monoclonal antibody to Hepatitis A virus capsid. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R10.
Immunogen: This antibody was raised by immunising BALB/c mice intraperitoneally with inactivated Hepatitis A viruses.
Specificity: This antibody potently and specifically binds to the surface of Hepatitis A viral particles, along the interfaces of the viral pentameric assemblies.
Application Notes: This neutralising antibody blocks Hepatitis A virus attachment to host cell receptors and interferes with viral uncoating (Wang et al, 2017). This antibody recognises conformational epitopes by ELISA, but not linear epitopes in immunoblot analysis (Wang et al, 2017). A Fab derived from this antibody has been used to determine the structure of Hepatitis A viral particles, through both cryo-EM microscopy and X-ray crystallography (Wang et al, 2017).
Antibody first published in:
Wang et al. Potent neutralization of hepatitis A virus reveals a receptor mimic mechanism and the receptor recognition site. Proc Natl Acad Sci U S A. 2017 Jan 24;114(4):770-775 PMID:28074040
Note on publication:
Describes the original generation of this antibody, and is characterisation in ELISA experiments and neutralisation assays.