United Kingdom (UK)
Recombinant monoclonal antibody to IL-10R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B1.3a.
UniProt Accession Number of Target Protein: Q61727
Alternative Name(s) of Target: CD210; Interleukin-10 receptor subunit; Interleukin-10 receptor subunit alpha; Il10ra Il10r; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; IBI-3A; 1B1-3A
Immunogen: This antibody was raised by immunising rats with the purified recombinant extracellular region of mouse Interleukin 10 Receptor.
Specificity: This antibody recognises mouse IL-10R which is a cell surface glycoprotein and a receprot for anti-inflammatory IL-10. The epitope recognised by this antibody has been localised to the ligand binding domain within the extracellular region of mouse IL-10R.
Application Notes: 1B1.3a has been widely applied in flow cytometric analyses where the detection of IL-10R was needed. Furthermore, this antibody was also successfully utilised as a blocking agent; one group used it to demonstrate that IL-10 mediates suppressive activity, by targeting its receptor IL-10R with this antibody (Wang et al., 2004). Castro et al. (2000) demonstrated that 1B1.3a is able to make a soluble peptide or protein antigen immunogenic for subsequent Th1 recall responses in a situation when there is an additional signal, such as LPS, activating the immune system. Consequently, this antibody is a hugely promising agent in the potential development of vaccines for immunotherapy. Similarly, Vicari and colleagues (2002) showed that this antibody has an enormous potential as a therapeutic anti-tumour agent when combined with the administration of CpG to stimulate the immune system.
Antibody first published in:
O'Farrell et al. IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways. EMBO J. 1998 Feb 16;17(4):1006-18. PMID:9463379
Note on publication:
This article describes the method of generation of antibodies like 1B1.3a.
Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a (Ab01490) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1B1.3a (Ab01490-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01490-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-IL-10R antibody 1B1.3a (Ab01490). RAW264.7 cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 1B1.3a (Ab01490-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.