United Kingdom (UK)
Recombinant monoclonal antibody to IRTA-5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2G5.
UniProt Accession Number of Target Protein: Q96LA6
Alternative Name(s) of Target: CD307a; Immune Receptor Translocation-Associated Protein 5; Fc Receptor Like 1; FCRL1; FcR-like protein 1; Fc receptor homolog 1; FcRH1
Immunogen: Mice of the HCo7 HuMab Mouse(R) strain were immunized with purified recombinant IRTA5 fusion protein. Mouse splenocytes, isolated from the HuMab mice, were fused with PEG to a mouse myeloma cell line. The resulting hybridomas were then screened for the production of antigen-specific antibodies. The 2G5 clone was selected from this.
Specificity: 2G5 is specific for IRTA5; it does not bind IRTA1, IRTA2, IRTA3, or IRTA4.
Application Notes: SPR was used to determine the Kd of 2G5 binding to IRTA5 as 0.028 x 10^-9 M. Using flow cytometry 2G5 was shown to be specific for IRTA5; it does not bind IRTA1, IRTA2, IRTA3, or IRTA4. Flow cytometry also showed that 2G5 directed against human IRTA-5 binds to CD 19+ B cells and the B-cell tumor lines Karpas 1106P, SU-DHL-4, Granta 519, and L-540. 2G5 does not bind CD3+ peripheral blood T cells, CD 1A+ peripheral blood dendritic cells, CD14+ peripheral blood monocytes, or CD56+ peripheral blood NK cells.
Antibody first published in: PMID: