United Kingdom (UK)
Recombinant monoclonal antibody to Insulin Receptor alpha. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 83-14.
UniProt Accession Number of Target Protein: P06213
Alternative Name(s) of Target: CD220; Insulin Receptor alpha; HHF5; IR; HIR
Immunogen: This antibody was raised by immunizing mice with IM-9 lymphocytes followed by purified insulin receptor.
Specificity: Recognises the extracellular domain of the Insulin Receptor alpha. Amino acids 469 - 592 (exon 7 and 8) of Insulin Receptor alpha.
Antibody first published in:
Soos MA, Siddle K, Baron MD, Heward JM, Luzio JP, Bellatin J, Lennox ES. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;235(1):199-208. PMID: 2427071
Note on publication:
Describes the immunisation of mice with IM-9 and/or purified Insulin receptor and the Identification of an Insulin Receptor alpha extracellular domain specific monoclonal antibody clone [83-14].
Flow-cytometry using the anti-Insulin Receptor alpha antibody 83-14 (Ab00137) Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (Ab00137-23.0, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.