United Kingdom (UK)
Recombinant monoclonal antibody to MARCO. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PLK1.
UniProt Accession Number of Target Protein: Q9UEW3
Alternative Name(s) of Target: SCARA2; macrophage receptor MARCO; macrophage receptor with collagenous structure; scavenger receptor class A member 2; PLK-1; PLK 1
Immunogen: The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.
Specificity: This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.
Application Notes: The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005; Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005).
Antibody first published in:
Arredouani et al. MARCO Is the Major Binding Receptor for Unopsonized Particles and Bacteria on Human Alveolar Macrophages J Immunol November 1, 2005, 175 (9) 6058-6064 PMID:16237101
Note on publication:
The article describes the method of generation of anti-MARCO PKL1 antibody and its effectivenes in IHC, IP and functional experiments.
Immunofluorescence staining of fixed Human peripheral blood leukocytes with anti-MARCO antibody PLK1 (Ab01384) Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of PLK1 (Ab01384-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01384-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.