Anti-NFKB2 [SAIC-26C-15]

Description:

Recombinant monoclonal antibody to NFKB2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-26C-15.

Clone Name:

SAIC-26C-15

Published Application(s):

immunoMRM

Published Species Reactivity:

Human

Products based on this clone:

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Ab00318-23.0 Anti-NFKB2 [SAIC-26C-15]
Human
Rabbit IgG
Purified
In Stock

Ab00318-1.1 Anti-NFKB2 [SAIC-26C-15]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: Q00653

Alternative Name(s) of Target: LYT10; Nuclear factor NF-kappa-B p100 subunit
Immunogen: Peptide "IEVDLVTHSDPPR" derived from the transcription factor NFKB2 (Nuclear factor NF-kappa-B p100 subunit) conjugated to KLH.

Specificity: Recognises human NFKB2.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

IF staining of HeLa cells using Ab00318-23.0. Click on image for more details.

Immunofluorescence staining of fixed HeLa cells with anti-NFKB2 antibody SAIC-26C-15 (Ab00318) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of SAIC-26C-15 (Ab00318-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining, most likely in the ER. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00318-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.

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