United Kingdom (UK)
Recombinant monoclonal antibody to Rad51. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3C10.
UniProt Accession Number of Target Protein: Q06609
Alternative Name(s) of Target: DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A; BRCC5; HsT16930; RAD51 homolog; homolog of RecA, E. coli; RecA-like protein; RAD51ABRCA1/BRCA2-containing complex, subunit 5; Ab1; Ab-1; Rad51 Ab1; 51RAD01
Immunogen: This antibody was raised against the recombinant human RAD51 protein.
Specificity: This antibody recognises RAD51 (and might also recognise hDmc1 (Masson et al., 1999)). RAD51 is a eukaryotic homologue of E. coli RecA. It is an important part of the homologous recombination DNA repair pathway during the homologous strand exchange stage.
Application Notes: WB: Anti-Rad51 3C10 antibody was used to visualise Rad51 via Western blot after previous immunoprecipitation with different antibodies in experiments analysing the interactions between Rad51 and DMC1 (Masson et al., 1999). IF: 3C10 antibody was used to visualise Rad51 on chromatin in endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). IHC: This antibody was also used to immunohistochemically stain biopsy and resected specimens of esophagus and squamous cell carcinoma of the esophagus (Nakanoko et al., 2014).
Antibody first published in:
Masson et al. The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51. EMBO J. 1999 Nov 15;18(22):6552-60. PMID:10562567
Note on publication:
This article describes the experiments for which anti-Rad51 3C10 antibody was created. It was used to visualise Rad51 via Western blot after previous immunoprecipitation with different antibodies.