United Kingdom (UK)
Recombinant monoclonal antibody to SIRP g. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-119.
UniProt Accession Number of Target Protein: Q9P1W8
Alternative Name(s) of Target: CD172g; OX119; MRC OX-119; MRC OX119; SIRP-g; SIRPg; SIRP gamma; SIRP-gamma; CD172 antigen-like family member B; signal-regulatory protein beta; CD172-g
Immunogen: OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).
Specificity: Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..
Application Notes: OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA.
Antibody first published in:
Brooke et al Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol 173:2562-70 2004 PMID:15294972
Note on publication:
Describes the determintion of OX-119 specificity by ELISA, FC and IP and decribes use of OX-119 to observe the distribution of SIRPg expression and its ability to block SIRPg-mediated apoptosis.
Flow-cytometry using the anti-SIRP g antibody OX-119 (Ab00589) Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of OX-119 (Ab00589-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.