Anti-SNCG [SAIC-31A-10]

Description:

Recombinant monoclonal antibody to SNCG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-31A-10.

Clone Name:

SAIC-31A-10

Published Application(s):

WB, ELISA, IHC

Published Species Reactivity:

Human

Products based on this clone:

{{heading}}

Please Select

Ab00324-23.0 Anti-SNCG [SAIC-31A-10]
Human
Rabbit IgG
Purified
In Stock

Ab00324-1.1 Anti-SNCG [SAIC-31A-10]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: Q6FHG5

Alternative Name(s) of Target: Synuclein gamma variant; Synuclein, gamma (Breast cancer-specific protein 1) variant
Immunogen: The original antibody was raised against the KLH-conjugated peptide "TVEEAENIAVTSGVVR", derived from Gamma-synuclein, which plays a role in neurofilament network integrity.

Specificity: This antibody recognizes human SNCG.

Application Notes: The original characterization data for this antibody can be found here.

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133 PMID:25512614
Note on publication: The original publication describes the generation of a panel of recombinant antibodies using synthetic peptides of human proteins as well as their validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of U251 cells using Ab00324-23.0. Click on image for more details.

Flow cytometry using the Anti-SNCG antibody SAIC-31A-10 (Ab00324). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (Ab00324-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of U251 cells using Ab00324-23.0. Click on image for more details.

Immunofluorescence staining of fixed U251 cells with anti-SNCG antibody SAIC-31A-10 (Ab00324). Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-31A-10 (Ab00324-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00324-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience.

Necessary

Always Enabled

Necessary cookies are absolutely essential for the website to function properly. These cookies ensure basic functionalities and security features of the website, anonymously.

Cookie	Duration	Description
ASP.NET_SessionId	session	Issued by Microsoft's ASP.NET Application, this cookie stores session data during a user's website visit.
cookielawinfo-checkbox-advertisement	1 year	Set by the GDPR Cookie Consent plugin, this cookie is used to record the user consent for the cookies in the "Advertisement" category .
cookielawinfo-checkbox-analytics	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checkbox-functional	11 months	The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checkbox-necessary	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-others	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-performance	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
viewed_cookie_policy	11 months	The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.

Analytics

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.

Cookie	Duration	Description
_dc_gtm_UA-49047364-1	1 minute	No description
_ga	2 years	The _ga cookie, installed by Google Analytics, calculates visitor, session and campaign data and also keeps track of site usage for the site's analytics report. The cookie stores information anonymously and assigns a randomly generated number to recognize unique visitors.
_gcl_au	3 months	Provided by Google Tag Manager to experiment advertisement efficiency of websites using their services.
_gid	1 day	Installed by Google Analytics, _gid cookie stores information on how visitors use a website, while also creating an analytics report of the website's performance. Some of the data that are collected include the number of visitors, their source, and the pages they visit anonymously.
ai_session	30 minutes	This is a unique anonymous session identifier cookie set by Microsoft Application Insights software to gather statistical usage and telemetry data for apps built on the Azure cloud platform.
ai_user	1 year	A unique user identifier cookie, set by Microsoft Application Insights software, that enables counting of the number of users accessing the application over time.

Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.

Cookie	Duration	Description
_uetsid	1 day	Bing Ads sets this cookie to engage with a user that has previously visited the website.
_uetvid	1 year 24 days	Bing Ads sets this cookie to engage with a user that has previously visited the website.
MUID	1 year 24 days	Bing sets this cookie to recognize unique web browsers visiting Microsoft sites. This cookie is used for advertising, site analytics, and other operations.
test_cookie	15 minutes	The test_cookie is set by doubleclick.net and is used to determine if the user's browser supports cookies.