United Kingdom (UK)
Recombinant monoclonal antibody to Thyroid peroxidase. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from cDNA library WR1.7.
UniProt Accession Number of Target Protein: P07202
Alternative Name(s) of Target: TPO
Immunogen: A cDNA library was constructed from the thyroid tissue of patients with Grave's disease, and antibody fragments were expressed in XL1-Blue cells.
Specificity: TPO is a cell surface enzyme responsible for thyroid hormone synthesis and present in virtually all patients with active autoimmune thyroid disease.
Application Notes: WR.1.7. binds human TPO with a Kd of 2.9 x 10^-10 M (Chazenbalk et al, 1993). Competition assays were used to characterise the WR1.7 eptitope with respect to other clones, identifying that WR1.7 binds a distinct eptitope to clone TR1.9 (Chazenbalk et al, 1993). WR1.7, TR1.9, SP1.4, and TR1.8 together map the immunodominant region of TPO recognised by autoantibodies in patients' sera (Nishikawa et al, 1994). WR1.7 was able to inhibit TPO autoantibodies in patients' sera as shown with further competition studies between antibody fragments and serum TPO autoantibodies (Chazenbalk et al, 1993). WR1.7 has been used to detect cells expressing cell surface TPO through flow cytometry (Jaume et al, 1999).
Antibody first published in:
Chazenbalk et al, 1993. Human organ-specific autoimmune disease. Molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ. J Clin Invest. 1993 Jul; 92(1): 62–74. PMID:7686925
Note on publication:
Describes the original production of the antibody and its characterisation through competition assays.