United Kingdom (UK)
Recombinant monoclonal antibody to beta 3 integrin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma EM00212.
UniProt Accession Number of Target Protein: P05106
Alternative Name(s) of Target: β3A; Platelet membrane glycoprotein IIIa; CD61; b3
Immunogen: This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas
Specificity: This antibody is specific for the cytoplasmic domain (amino acids 742 to 788) of integrin β3.
Application Notes: This antibody has been used to stain β3-containing integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014).
Antibody first published in:
Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423
Note on publication:
Describes the generation and characterisation of this antibody.