United Kingdom (UK)
Recombinant monoclonal antibody to α-gal . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma B4H2.
Alternative Name(s) of Target: alpha gal; Galα1–3Galβ1–4GlcNAc-R; gal
Immunogen: This antibody was raised by immunising α1,3galactosyltransferase knock-out mice, which lack α-gal epitopes. Mice were immunised with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
Specificity: This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycoprotein and glycolipid molecules.
Application Notes: This antibody binds to glycoprotein and glycolipid molecules expressing α-gal epitopes, including α-gal-BSA, in ELISA analysis (Chen et al, 2005). It does not cross-react with any glycoproteins lacking this epitope. This antibody binds to the α-gal epitopes on the hybridoma cells producing the antibody, so can mediate complement mediated autolysis in the presence of rabbit serum (Chen et al, 2000).
Antibody first published in:
Chen et al. Genes coding evolutionary novel anti-carbohydrate antibodies: studies on anti-Gal production in α1,3galactosyltransferase knock out mice Molecular Immunology, 2000, Vol.37(8), pp.455-466 PMID:11090880
Note on publication:
Describes the original generation of this antibody, and its characterisation in ELISA analysis.