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- Ab01159-1.1 Anti-1-4 beta-d-galactan [XD3]
- Zinnia elegans
- Mouse IgG1
- Purified
- Ships in 5-6 weeks
- Ab01159-23.0 Anti-1-4 beta-d-galactan [XD3]
- Zinnia elegans
- Rabbit IgG
- Purified
- Ships in 5-6 weeks
Recombinant monoclonal antibody to 1-4 beta-d-galactan. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody XD3.
Alternative Name(s) of Target: 14bdGal; (1→4)-β-d-galactan
Immunogen: This antibody was isolated from a phage-display library raised against a cell wall fraction of Zinnia elegans xylogenic culture cells. Cell wall fractions were isolated from Zinnia mesophyll cells that had been cultured either in a differentiation-inducti
Specificity: This antibody recognises (1→4)-β-d-galactan. The epitope recognised is present on the primary wall of tracheary-element precursor cells, but absent from mature tracheary elements.
Application Notes: Whole-cell ELISA against cultured plant cells has confirmed that this antibody is differentiation-specific, as the epitope recognised by this antibody increased specifically on cells cultured in a differentiation-inductive medium but not on those cultured in a noninductive medium (Shinohara et al, 2002).Equally, this antibody has been shown in ELISA analysis to recognise (1→4)-β-d-galactan in a pectic fraction (Shinohara et al, 2015).
Antibody first published in:
Shinohara et al. Isolation of monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction J Immunol Methods. 2002 Jun 1;264(1-2):187-94. PMID:12191521
Note on publication:
Describes the original generation of this antibody, and its characterisation in ELISA analysis.