United Kingdom (UK)
Recombinant monoclonal antibody to 1-pHis. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SC50-3.
Alternative Name(s) of Target: 1-phosphohistidine; N1-Phosphohistidine; histidine
Immunogen: The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).
Specificity: This antibody is specific for 1-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.
Application Notes: This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. A dot blot was preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating its specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). This antibody has been also used for a western blot on His6-NDPK-A protein (Felix et al, 2020; pmid:32384736). Immunofluorescence was preformed on mouse tissues with this antibody (Yang et al, 2019; pmid:31721359). An ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). Ca1+-VPCs were lysed and immunoprecipitated with this clone (Yang et al, 2019; pmid:31721359). Finally, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889).
Antibody first published in:
Fuhs et al. Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1): 198–210. PMID:26140597
Note on publication:
Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis.