United Kingdom (UK)
Recombinant monoclonal antibody to 2.4[2.8]2.4-linked Kdo tetrasaccharide. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma S73-2.
Immunogen: This antibody was raised by immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA and further immunizing them with Kdo(2f8)[Kdo(2f4)]Kdo-BSA.
Specificity: This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).
Application Notes: To screen hybridomas for this antibody ELISA was performed on neoglycoconjugates and immunofluorescence was performed on cells infected with either C. trachomatis or C. psittaci. Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650, and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a reference protein using the mouse version of this antibody (Brooks et al, 2010; pmid:20000757).
Antibody first published in:
Brooks et al. Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry. 2010 Jan 26;49(3):570-81. PMID:20000757
Note on publication:
The structures of antigen-binding fragments from two related monoclonal antibodies have been determined to high resolution in the presence of several carbohydrate antigens raised against chlamydial lipopolysaccharide.