United Kingdom (UK)
Recombinant monoclonal antibody to 3–5 ring polycyclic aromatic hydrocarbons (PAHs). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2G8.
Alternative Name(s) of Target: unsubstituted PAH; 2-methylphenanthrene; 9,10-dimethylanthracene; 7,12 dimethybenz(a)anthracene; 4-methyl dibenzothiophene; Benzo(a)pyrene; Chrysene; Pyrene; Phenanthrene; Anthracene
Immunogen: The original antibody was generated by immunizing Balb/c mice were with 1-pyrene butyric acid-KLH.
Specificity: The antibody shows general selectivity and high sensitivity to a variety of 3–5 ring PAH, but it shows a low affinity for 2-ring PAHs. Polycyclic aromatic hydrocarbons (PAHs), a group of organic compounds composed of two or more blended aromatic rings, are an important class of environmental pollutants
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody could inhibit unsubstituted and methylated PAHs by indirect competitive ELISA. IC50 values for unsubstituted PAHs ranged from 1.68 μg/L to 9.44 μg/L, while IC50 values against methylated PAHs (2-methylphenanthrene, 9,10-dimethylanthracene, 7,12 dimethybenz(a)anthracene, 4-methyl dibenzothiophene) were ranging from 1.92 μg/L to 31 μg/L. The antibody was employed in the KinExA Inline Biosensor system for quantifying 3–5 ring PAHs in aqueous environmental samples (detection limit 0.2 μg/L) (Li et al., 2016; PMID: 26925369).
Antibody first published in:
Li et al. A highly sensitive monoclonal antibody based biosensor for quantifying 3–5 ring polycyclic aromatic hydrocarbons (PAHs) in aqueous environmental samples. Sens Biosensing Res. 2016 Mar 1;7:115-120. PMID:26925369
Note on publication:
The original paper describes the generation and characterization of the antibody.