United Kingdom (UK)
Recombinant monoclonal antibody to 3-Phenoxybenzoic acid. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3P5ThC12.
Alternative Name(s) of Target: 3-PBA; 3739-38-6; m-Phenoxybenzoic acid; 3-Carboxydiphenyl ether
Immunogen: The original antibody was generated by immunizing an alpaca with 3-PBA, followed by antibody library construction and phage display-based selection.
Specificity: The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3-Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfenvalerate, Deltamethrin, Cyfluthurin. 3-PBA is a human urinary metabolite of pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides.
Application Notes: The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. The cd-FEIA showed 50% inhibitory concentration (IC50) and the linear range of respectively 0.082 and 0.015-0.447 ng/ml, and a detection limit of 0.011 ng/ml (Huo et al., 2018; PMID: 30293433). The VHH fragment was employed to develop a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-PBA, showing a limit of detection of 3ng/ml (Riangrungro et al. 2019; PMID: 31462650).
Antibody first published in:
Kim et al. Isolation of alpaca anti-hapten heavy chain single domain antibodies for development of sensitive immunoassay Anal Chem. 2012 Jan 17;84(2):1165-71 PMID:22148739
Note on publication:
The paper describes the generation and characterization of the antibody.