United Kingdom (UK)
Recombinant monoclonal antibody to 4-1BB. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from The phage display antibody 3H3.
UniProt Accession Number of Target Protein: P20334
Alternative Name(s) of Target: CD137; 4 1BB; 41BB; Tumor necrosis factor receptor superfamily member 9; Tnfrsf9; T-cell antigen 4-1BB; Ila, Ly63; 4-1BB receptor
Immunogen: This antibody was raised by immunizing Lewis rats with 4-1BB fusion proteins, composed of the extracellular domain of murine 4-1BB and the COOH region of human IgG1 .
Specificity: This antibody is specific for murine 4-1BB, an inducible type I membrane protein that is rapidly expressed on the surface of CD4+ and CD8+ T cells after activation.
Application Notes: The binding specificity of this antibody for 4-1BB has been confirmed in ELISA analysis using purified 4-1BB–Ig fusion protein, and immunoprecipitation analysis (Shuford et al, 1997).This antibody is able to block 4-1BBL binding to activated DO.11.10 T cells (Shuford et al, 1997). This antibody reacts with activated T cells, and can costimulate T cell proliferation induced by the 145.2C11 hamster anti– mouse CD3 mAb (Shuford et al, 1997). Equally, when administered to mice, this antibody expands innate immune cells, as well as host T cells (Lin et al, 2010). Administration of this antibody alone induced complete tumor regression in E.G7 tumor-bearing mice (Lin et al, 2010). Binding of this antibody to activated T cells in mice receiving heart allografts led to the rapid rejection of the allograft (Shuford et al, 1997).
Antibody first published in:
Shuford et al. 4-1BB costimulatory signals preferentially induce CD8+ T cell proliferation and lead to the amplification in vivo of cytotoxic T cell responses. J Exp Med. 1997 Jul 7;186(1):47-55. PMID:9206996
Note on publication:
Describes the original generation of this antibody, and its characterisation in ELISA analysis.