United Kingdom (UK)
Recombinant monoclonal antibody to 4-1BB. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BBK2 .
UniProt Accession Number of Target Protein: Q07011
Alternative Name(s) of Target: CD137; MGC2172; Tumor necrosis factor receptor superfamily member 9; TNFRSF9; T cell antigen ILA; Interleukin activated receptor; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; BBK-2
Immunogen: The original antibody was raised by immunizing a mouse with CD137.
Specificity: This antibody is specific for human CD137.
Application Notes: An in vitro T cell killing assay was performed using this antibody (US20200407440). Immunofluorescence was performed on KM-H2 cells using this antibody (Ho et al, 2013; pmid:23204227). Flow cytometry was performed with this antibody on cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). Immunoprecipitation was performed with this antibody on the cell lines: KM-H2, L-428, L-1236 KM-H2-control, KM-H2-CD137 and stable cell lines expressing CD137 or CD137L (Ho et al, 2013; pmid:23204227). This antibody was used for immunohistochemistry on human tissue from lung, liver, colon, soft tissue, thyroid gland, stomach, parathyroid gland, and spleen, parotid gland, heart, aorta, kidney, and lymph node (Broll et al, 2001; pmid:11293902). Human lymph node was used for immunohistochemistry using this antibody (Pauly et al, 2002; pmid:12101260). Flow cytometry was performed on cell line FDC-H1, Human peripheral blood mononuclear cells and B lymphocytes using this antibody (Pauly et al, 2002; pmid:12101260). This antibody was used for immunohistochemistry on human tissue (Drenkard et al, 2007; pmid:17167064). Blocking of the CD137-fc protein was tested in MoDC cells using this antibody (Lippert et al, 2008; pmid:18395851).
Antibody first published in:
Broll et al. CD137 expression in tumor vessel walls. High correlation with malignant tumors. Am J Clin Pathol. 2001 Apr;115(4):543-9. PMID:11293902
Note on publication:
Immunohistochemical studies were used on human tissue samples to determine in vivo CD137 expression in nonimmune tissue samples.