- Ab00644-1.1 Anti-alpha-4 integrin [21.6]
- Mouse IgG1
- In Stock
- Ab00644-23.0 Anti-alpha-4 integrin [21.6]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to alpha-4 integrin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 21.6.
UniProt Accession Number of Target Protein: P13612
Alternative Name(s) of Target: CD49D; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha
Immunogen: Alpha-4 integrin.
Specificity: This antibody binds to human alpha-4-integrin
Application Notes: This antibody binds to human alpha-4-integrin and can inhibit the binding of lymphocytes to endothelial cells, suppressing inflammation.
Antibody first published in: Methods of inhibiting alpha-4-dependent interactions with VCAM-1 with anti-VLA-4 antibodies Patent US8246958 PMID: Note on publication: Describes the use of this murine antibody and humanized counterpart, their affinities and biological function.
Immunofluorescence staining of fixed K562 cells with anti-alpha-4 integrin antibody 21.6 (Ab00644) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 (Ab00644-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00644-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.