Anti-Asp f 1 [4F8]

Description:

Recombinant monoclonal antibody to Asp f 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4F8.

Clone Name:

4F8

Published Application(s):

IP, WB, ELISA

Published Species Reactivity:

Aspergillus fumigatus (Neosartorya fumigata)

5

Products based on this clone:

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Ab01405-14.0 Anti-Asp f 1 [4F8]
Aspergillus fumigatus (Neosartorya fumigata)
Human IgE
Purified
In Stock

Ab01405-10.0 Anti-Asp f 1 [4F8]
Aspergillus fumigatus (Neosartorya fumigata)
Human IgG1
Purified
Ships in 4-5 weeks

Ab01405-1.1 Anti-Asp f 1 [4F8]
Aspergillus fumigatus (Neosartorya fumigata)
Mouse IgG1
Purified
Ships in 4-5 weeks

Ab01405-20.0 Anti-Asp f 1 [4F8]
Aspergillus fumigatus (Neosartorya fumigata)
Mouse IgE
Purified
Ships in 4-5 weeks

Ab01405-23.0 Anti-Asp f 1 [4F8]
Aspergillus fumigatus (Neosartorya fumigata)
Rabbit IgG
Purified
In Stock

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UniProt Accession Number of Target Protein: P67875

Alternative Name(s) of Target: A. fumigatus Allergen; Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1
Immunogen: 4F8 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion. Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE

Specificity: This antibody recognises Asp f 1.

Application Notes: This antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB in ELISA and western blot (Wurth et al., 2018).

Antibody first published in: Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320
Note on publication: The paper describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.