Anti-Band 3/CD233 [Q1/156]

Description:

Recombinant monoclonal antibody to Band 3/CD233. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Q1/156.

Developed in partnership with Ximbio (www.ximbio.com).

Clone Name:

Q1/156

Published Application(s):

IP, WB, IF, IHC

Published Species Reactivity:

Human

5

Products based on this clone:

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Ab01345-1.1 Anti-Band 3/CD233 [Q1/156]
Human
Mouse IgG1
Purified
In Stock

Ab01345-23.0 Anti-Band 3/CD233 [Q1/156]
Human
Rabbit IgG
Purified
In Stock

Ab01345-10.0 Anti-Band 3/CD233 [Q1/156]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01345-10.3 Anti-Band 3/CD233 [Q1/156]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01345-13.12 Anti-Band 3/CD233 [Q1/156]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

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UniProt Accession Number of Target Protein: P02730

Alternative Name(s) of Target: Leu2;Band 3 anion transport protein; Band III; Anion exchange protein 1; AE 1; Anion exchanger 1; Solute carrier family 4 member 1; BND3; CD233 antigen; erythrocyte membrane protein band 3; Solute carrier family 4 member 1; FR; WD; RTA1A; AE1
Immunogen: This antibody was raised by immunising mice with fetal liver cells.

Specificity: This antibody recognises Band 3 protein which is both an anion transporter and a primary structural protein in erythrocytes. Mutations in its gene lead to a number of hemolytic anemias.

Application Notes: Band 3 is a primary, most abundant protein in the membrane of erythrocytes and Q1/156 antibody is recommended as a red blood cell marker. This antibody was used to immunoprecipitate crosslinked band 3 protein complexes as well as to deterime which oligomers were the most prevalent via Western blot (Rivera-Santiago et al., 2017). This antibody was also utilised in the identification of erythrocytes via immunohistochemistry to differentiate between the cells of various developmental stages (Watt et al., 1998).

Antibody first published in: Majdic et al. Comparative evaluation of CD10 antibodies. In McMichael AJ, et al (eds) Leucocyte Typing III, Oxford University Press, Oxford, New York and Tokyo, p 488-9. PMID:

Binding Curve

IF analysis of K562 cells using Ab01345-1.1. Click on image for more details.

Binding Curve

Immunofluorescence staining of K562 cells using anti-Band 3/CD233 (Ab01345) Q1/156 Immunofluorescence analysis of paraformaldehyde fixed K562 cells stained with the chimeric mouse IgG version of Q1/156 (Ab01345-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01215-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.

Binding Curve

Flow cytometry analysis of K562 cells using Ab01345-1.1 Click on image for more details.

Binding Curve

Flow cytometry using the Anti-Band 3/CD233 antibody Q1/156 (Ab01345). Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of Q1/156 (Ab01345-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.