- Ab01322-1.1 Anti-BLC2 [bcl-2/124]
- Mouse IgG1
- In Stock
- Ab01322-23.0 Anti-BLC2 [bcl-2/124]
- Rabbit IgG
- In Stock
- Ab01322-10.0 Anti-BLC2 [bcl-2/124]
- Human IgG1
- Ships in 4-5 weeks
Recombinant monoclonal antibody to BLC2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma bcl-2/124.
UniProt Accession Number of Target Protein: P10415
Alternative Name(s) of Target: Apoptosis regulator Bcl-2; AW986256; B-cell CLL/lymphoma 2; Bcl-2; C430015F12Rik; D630044D05Rik; D830018M01Rik; protein phosphatase 1; regulatory subunit 50; bcl-2-124; bcl2/124
Immunogen: This antibody was raised against a synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein.
Specificity: This antibody recognises human BLC-2 which is an antiapoptotic factor in various cell types. Its disregulation was implicated in different conditions, such as cancers and autoimmune disorders.
Application Notes: This antibody has been used extensively in attempts aimed at characterising various cancer tissues via immunohistochemical staining. One group used bcl-2-124 antibody to label large B-cell lymphoma samples for bcl2 in order to perform its immunophenotypic and genetic depiction (Chang et al., 2016). Another group took advantage of this antibody while analysing the molecular picture of a case of the cystic synovial sarcoma of the pleura where they observed positive immunohistochemical staining in tumor cells (Tajima et al., 2015). Bcl-2 plays an important role as an inhibitor of apoptosis and its identification is often useful while analysing various factors influencing apoptosis. For instance, bcl-2/124 antibody has been successfully utilised in such experiments identifying bcl-2 via Western blot - Gomez-Bougie (2007) probed the influence of bortezomib on HMCL cells and detected bcl-2 signal on Western blots.
Antibody first published in: Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990 Aug;137(2):225-32. PMID:2201196 Note on publication: This article describes generation and characterisation of the bcl-2/124 antibody.
Immunofluorescence staining of Jurkat cells using anti-BLC2 antibody (Ab01322) bcl-2/124. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG version of bcl-2/124 (Ab01322-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01322-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488
Flow-cytometry using the anti-BLC2 antibody bcl-2/124 (Ab01322). Jurkat cells were fixed using paraformaldehyde, permeabilised using 0.5% Triton and stained with anti-unknown IgG antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG-chimeric version of bcl-2/124 (Ab01322-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-BCL2 antibody bcl-2/124 (Ab01322). Jurkat(A) (0.01 µg/ml), U937(B) (0.01 µg/ml), K562(C) (0.03µg/ml), HeLa(D) cells (0.1 µg/ml), human spleen(E) (0.3 µg/ml), human thymus(F) (0.03µg/ml) and human tonsil(G) (0.03 µg/ml) tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of bcl-2/124 (Ab01322-1.1) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the Anti-BLC2 antibody bcl-2/124 (Ab01322). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of bcl-2/124 (Ab01322-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.