United Kingdom (UK)
Recombinant monoclonal antibody to Bovine Herpesvirus gD. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BoScFv.
UniProt Accession Number of Target Protein: P0CK30
Alternative Name(s) of Target: BoHV-1 gD; gd; Envelope glycoprotein D; Bovine herpesvirus glycoprotein D; Glycoprotein IV; gIV; US6
Immunogen: The original antibody was isolated by screening hybridomas against 'MEESKGYEPP' of the bovine gD.
Specificity: This antibody binds the amino acid residues 'EESKGYEPP' of the bovine herpervirus glycoprotein D.
Application Notes: Western blot and ELISA analysis showed that this scFv had a high affinity for BoHV-1 gD, with a Kd of 161.2 ± 37.58 nM and for whole BoHV-1 virus, with a Kd of 67.44 ± 16.99 nM. This scFv displayed a high affinity for BoHV-1 antigen in an ELISA and competed with BoHV-1 anti-serum in a competitive ELISA. Immunofluorescence assay (IFA) and laser confocal microscopy showed that this scFv could efficiently bind to and be internalized by BoHV-1 infected Madin-Darby bovine kidney (MDBK) cells. This scFv was shown to inhibit BoHV-1 infectivity and to reduce the number of viral plaques by blocking viral attachment to MDBK cells (PMID: 29063173). The recombinant BoScFv-PE38 immunotoxin expressed in a prokaryotic expression system has specific binding affinity for BoHV-1 glycoprotein D (gD) with a dissociation constant (Kd) of 12.81 nM and for BoHV-1 virus particles with a Kd value of 97.63 nM. BoScFv-PE38 exerted a cytotoxic effect through the induction of ATP and ammonia, leading to apoptosis of BoHV-1-infected cells and the inhibition of BoHV-1 replication in MDBK cells (PMID: 29670605).
Antibody first published in:
Xu et al. Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Appl Microbiol Biotechnol (2017); 101(23-24):8331-8344. PMID:29063173
Note on publication:
Appl Microbiol Biotechnol (2017); 101(23-24):8331-8344.